Efficacy of a Biorevitalizing-Filler Solution on All Skin Aspects: 10 Years Approach through <i>in Vitro</i> Studies and Clinical Trials

Introduction: Skin aging is the result of many cellular dysfunctions over time particularly fibroblasts and keratinocytes. These could be decelerated by preventive effects some skin treatments such as Intradermal injections. NCTF 135HA has a polycomponent formulation designed to improve efficacy non-cross linked hyaluronic acid (as micro-filler) on function. Although been used aesthetic practitioners since 20 years, we have analyzed all in vitro, ex vivo studies during past 10 years order summarized its anti-aging effect. Methods: In these series studies, known HA evaluated with this boosted formula. Collagen I synthesis, antiglycation effect contractile forces developed were studied. Fibroblasts keratinocytes proliferations monolayer cells culture. The filling thanks injection was performed fringe projection protective against solar irradiation study proliferation rate, elastin collagen expression. Two clinical trials women evaluate injection. Hydration, firmness, radiance, wrinkles size, pore thickness density dermis analyzed. Results: I, anti-AGE products, cell significantly increased culture medium. Ex showed that increased, production. addition, superior placebo. first trial shows tonicity, hydration radiance decreased wrinkle score. second demonstrated thickness, homogeneity size depth volume. Conclusion: minimally invasive technique quality which visual preventing signs.